Coding

Part:BBa_K3260028:Design

Designed by: Mary Elizabeth Adler   Group: iGEM19_BrownStanfordPrinctn   (2019-10-14)


cGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was incorporated into fusion proteins using a flex linker BBa_K3260026.


Source

The position of the split was informed by Magliery et. all in their 2004 paper on GFP fragment reassembly. The GFP sequence was taken from Uniprot P42212.

References